Interaction of Antibiotics with Deoxyribonucleic Acid

Publisher Website
Google Scholar
Abstract
The interactions of several antibiotics with deoxyribonucleic acid were studied by the sensitivities of their complexes to nucleolytic enzymes. After treatment with mitomycin, DNA was hydrolyzed slower than normal DNA with make venom phosphodiesterase [EC 3.1.4.1] but at the same rate with pancreatic DNase I [EC 3.1.4.5]. Actinomycin D, quinomycin A, triostin C and chromomycin A3 strongly inhibited the digestion of DNA with DNase I, chromomycin being the strongest inhibitor. The interactions of these drugs with DNA were studied by spectrophotometric titration. In general it was found that the sensitivity to DNase decreases by half when the binding sites are just filled by these drugs.

This publication has 0 references indexed in Scilit: