Compaction-defective embryonal carcinoma cell variants

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Abstract
Compaction of the morula is a prerequisite for subsequent differentiation of the mouse embryo. Analogous differentiation follows compaction in aggregates of several embryonal carcinoma cell lines. This report describes the isolation of two compaction‐defective variants from the H6 embryonal carcinoma cell line. These were isolated directly as clonal compaction‐defective aggregates in medium containing 1.3% methylcel‐lulose. They were obtained following chemical mutagenesis, since spontaneous variants were not seen. Compaction‐defective variants of the F9 ECC line or the ES‐D3 embryonic stem cell line could not be obtained. One of the H6 compaction‐defective variants appeared to be dominant when hybridized to its parental line, while the other appeared to be recessive.