DIVALENT CATIONS INCREASE [3H]‐PROSTACYCLIN BINDING TO MEMBRANES OF NEURONAL SOMATIC HYBRID CELLS
Open Access
- 19 July 1981
- journal article
- Published by Wiley in British Journal of Pharmacology
- Vol. 73 (3) , 691-694
- https://doi.org/10.1111/j.1476-5381.1981.tb16804.x
Abstract
1 [3H]-prostacyclin binding to membranes of a highly differentiated neuronal somatic hybrid is increased equally in the presence of Ca2+, Ba2+, Mg2+ or Sr2+ ions. 2 Analysis of the binding of [3H]-prostacyclin in the presence of low (1 mm) or high (50mm) Mg2+ ion concentrations has revealed a cation-dependent increase in ligand receptor affinity (Kd− values = 57.4 nM and 21.9 nM). 3 The increase in [3H]-prostacyclin binding due to divalent cations is not accompanied by an alteration in the maximum binding capacity of the membranes.Keywords
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