STUDIES ON BIOSYNTHESIS OF THE LINKAGE REGION OF CHONDROITIN SULFATE-PROTEIN COMPLEX
- 1 December 1966
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 56 (6) , 1859-1866
- https://doi.org/10.1073/pnas.56.6.1859
Abstract
A cell-free preparation from embryonic chick cartilage catalyzes the incorporation of xylose-C14 and galactose-c14 from the corresponding UDP derivatives into TCA [ trichloroacetic acid]-preciptable material. The xylose is incorporated without modification and can be entirely solubilized by treatment with alkaline borohydride yielding xylitol-C14. Xylosyl-C14-serine was isolated after extensive proteolytic digestion. Free galactose-C-14 can be recovered following acid and B-galactosidase hydrolysis of radioactive products isolated by alkali treatment of gal-actose-C14-labeled TCA precipitates. It is proposed that these results reflect the biosynthesis of the linkage region of CS [chondroitin sulfate] to protein.This publication has 21 references indexed in Scilit:
- Transfer of N-acetylneuraminic acid to incomplete glycoproteins associated with microsomesBiochimica et Biophysica Acta (BBA) - General Subjects, 1966
- Incorporation of D-galactose into liver microsomal protein in vitroBiochemical and Biophysical Research Communications, 1966
- Incorporation of D-xylose-C14 into glycoprotein by particles from hen oviductBiochemical and Biophysical Research Communications, 1966
- The Chemistry of Glomerular Basement Membrane and Its Relation to Collagen*Biochemistry, 1966
- Biosynthesis of Uridine Diphosphate D-Xylose. II. Uridine Diphosphate D-Glucuronate Carboxy-lyase of Cryptococcus laurentii*Biochemistry, 1966
- The biosynthesis of chondroitin sulfateArchives of Biochemistry and Biophysics, 1966
- Glycosylation of serine residues by a uridine diphosphate-xylose: Protein xylosyltransferase from mouse mastocytomaArchives of Biochemistry and Biophysics, 1966
- Decarboxylation of uridine diphosphate--glucuronic acid by an enzyme preparation from hen oviductBiochemical and Biophysical Research Communications, 1965
- Biosynthesis of bacterial lipopolysaccharide. V. Lipid-linked intermediates in the biosynthesis of the O-antigen groups of Salmonella typhimurium.Proceedings of the National Academy of Sciences, 1965
- LIPID-PHOSPHOACETYLMURAMYL-PENTAPEPTIDE AND LIPID-PHOSPHODISACCHARIDE-PENTAPEPTIDE: PRESUMED MEMBRANE TRANSPORT INTERMEDIATES IN CELL WALL SYNTHESISProceedings of the National Academy of Sciences, 1965