N‐terminal amino acid sequence of a major allergen of Japanese cedar pollen (Cry j I)
Open Access
- 7 November 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 239 (2) , 329-332
- https://doi.org/10.1016/0014-5793(88)80945-1
Abstract
A purified preparation of a major allergen of Japanese cedar pollen, sugi basic protein (SBP, Cry jI), was separated into 5 subtractions of 50‐45 kDa. All of the SBP subtractions were confirmed to be reactive to IgE antibodies from patients with Japanese cedar pollinosis, and also to mouse anti‐SBP monoclonal antibodies. The sequences of 20 N‐terminal amino acids of these 5 subtractions were found to be identical. Peptide mapping analyses of the SBP subtractions showed similar patterns, with some differences which might in part be due to the existence of an N‐linked carbohydrate chain. The N‐terminal amino acid sequence of SBP was identical to the reported sequence of an allergen of mountain cedar which vegetated in North America.Keywords
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