Fluorometric Method for Assay of Kallikrein-like Arginine Esterases
Open Access
- 1 June 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 79 (6) , 1197-1200
- https://doi.org/10.1093/oxfordjournals.jbchem.a131174
Abstract
The fluorometric method of Nash for determination of methanol was applied in assay of kallikrein-like arginine esterases using N-α-tosyl-L-arginine methyl ester (TAME) as substrate. The results obtained using this method corresponded well with those obtained in the usual colorimetric methods using hydroxamate, chromotropic acid, and 3-methyl-2-benzothiazolone hydrazone and the present method was more sensitive than these colorimetric methods.Keywords
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