STUDIES ON IMMUNE-RESPONSE TO FIXED ANTIGENS .3. INDUCTION OF HELPER FUNCTION FOR ANTIBODY-DEPENDENT CELLULAR CYTOTOXICITY RESPONSES

  • 1 January 1978
    • journal article
    • research article
    • Vol. 121  (2) , 401-408
Abstract
Heavy trinitrophenylated sheep cell red cells (TNP128SRC) and glutaraldehyde-treated SRC (G-SRC) could not induce cellular cytotoxicity against 51Cr-SRC. The native antigen SRC stimulated a cytolytic response against the radiolabeled homologous target cell. However, fixed SRC stimulated a priming function that accelerated and augmented the secondary cytotoxic response to SRC. Such fixed antigens stimulated a delayed-type hypersensitivity (DTHS) response also. Thus, the immunologic memory to the chemically modified antigen, as well as the DTHS response, are completely dissociated from the primary cytotoxic responses. The primary and the secondary cytotoxic responses that were developed in the spleens of the injected mice were mediated by antibody-dependent cellular cytotoxicity (ADCC), since the active supernatant that was released from the spleen cells lysed target cells in the presence of normal splenocytes. The active supernatant was identified as antibody. B [bone marrow derived] effector cells probably cytolyzed the antibody-coated target cells. Normal cells from nude mice could mediate the cytolytic process as efficiently as spleen cells from other strains of mouse. The results are discussed in terms of selective stimulation of T [thymus derived] cell subpopulations.

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