Radioimmunoassay of human calcitonin using labeled [Asu1,7]-human calcitonin analogue.
- 1 January 1978
- journal article
- research article
- Published by Japan Endocrine Society in Endocrinologia Japonica
- Vol. 25 (6) , 617-622
- https://doi.org/10.1507/endocrj1954.25.617
Abstract
Application of synthetic human calcitonin analogue ([Asu1, 7]-hCT) was attempted, since it is chemically more stable than native hCT.[Asu1, 7]-hCT was successfully labeled with 125I to a specific activity ranging from 410 to 540μCi/μg. Sensitivity, recovery and reproducibility of the assay system using 125I-labeled [Asul, 7]-hCT analogue, standard synthetic hCT and its antibodies, were 49pg/ml, 99.8±4%, and 7.95%(C. V. within assay) or 21.9%(C. V. between assays), respectively. The dilution curve of the sera obtained from patients with medullary carcinoma paralled well with the standard curve. 125I-labeled [Asul, 7]-hCT analogue can be used for routine radioimmunoassay of hCT.This publication has 1 reference indexed in Scilit:
- Comparison of Serum Calcitonin Levels After a 1-Minute Calcium Injection and After Pentagastrin Injection in the Diagnosis of Medullary Thyroid CarcinomaJournal of Clinical Endocrinology & Metabolism, 1977