The Purification of Prostatic Acid Phosphatase from Seminal Plasma by Reverse Phase High Pressure Liquid Chromatography

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Abstract
We report here the rapid isolation of PAP directly from dialyzed human seminal plasma by reverse phase high performance liquid chromatography (RP-HPLC). The recovery of PAP of high specific activity was found to be dependent on the treatment of the fractions after separation. The collection of fractions into a stabilizing medium was essential to the preservation of the specific activity of the enzyme. This finding extends the use of RP-HPLC to the purification of active enzymes from complex biological matrixes.