The production of ?-amylase in batch and chemostat culture by Bacillus stearothermophilus
- 1 January 1980
- journal article
- Published by Springer Nature in Antonie van Leeuwenhoek
- Vol. 46 (4) , 391-398
- https://doi.org/10.1007/bf00421985
Abstract
The production of α-amylase (α-1,4-glucan 4-glucanohydrolase; EC. 3.2.1.1) by a strain of Bacillus stearothermophilus isolated from leaf litter was investigated in a tryptone-maltose medium at 55°C in batch and chemostat culture. Amylase production was growth-limited and restricted to the exponential phase in batch culture. The enzyme yield was reduced by 40% when the culture pH was maintained at pH 7.2. Amylase production in chemostat culture was influenced by the growth rate throughout the dilution rate range used.Keywords
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