FLUORIMETRIC ESTIMATION OF OESTROGEN IN THE BLOOD OF INFANT FEMALE RATS

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Abstract
The development of characteristic castration changes in the anterior pituitary of rats ovariectomized at the age of 1 and 5 days (Presl, Horský, Henzl & Jirásek, 1963) means that the feedback mechanism between the ovaries and the diencephalohypophysial system becomes established early postnatally, before sexual maturity. The observed changes suggested that the ovaries already produce oestrogens during this period. The purpose of the present work was to obtain direct evidence of oestrogen production in 10-day-old female rats, i.e. when steroid-3β-ol dehydrogenase is already present in the ovaries (Presl, Jirásek, Horský & Henzl, 1965). Wistar rats bred in our laboratory were used. The animals were given heparin s.c. (10-day-old animals received 500 i.u., all others 1000 i.u.) and were killed by decapitation 45 min. later. Ovariectomized animals were killed 4 weeks after the operation. Adrenalectomy was performed 3 weeks after ovariectomy and the animals were killed 10 days later. For each

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