Protein synthesis by bovine chorion and uterine endometrium during the period of implantation

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Abstract
A grid method for culturing bovine endometrial and chorion tissue is described. A peak of MW 85,000 was obtained only from chorion, which was cultured with intercotyledonary tissue but the co-culture of cotyledon intercotyledon, kidney or liver with chorion obtained from cows 24-29 days pregnant did not significantly alter the overall protein synthesis level. The addition of progesterone or estradiol-17.beta. to the culture medium (10-4 mol) significantly depressed protein synthesis by both chorion and uterine endometrium.