Partial Purification and Antiserum Production to the 19 × 50-nm Mushroom Virus Particle

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Abstract
The 19 .times. 50 nm virus particles were purified from infected sporophores collected from mushroom [Agaricus bisporus] beds exhibiting characteristic disease symptoms. Sporophores were frozen in liquid N and stored at -20.degree. C. The polyhedral virus particles were removed by differential precipitation with 10% polyethylene glycol and 0.6 M NaCl. Sucrose density gradient centrifugation resulted in 1 major peak containing the 19 .times. 50 nm particles which exhibited an UV absorbance spectrum characteristic of a nucleoprotein with a 260/280 ratio of 1.4. Antiserum prepared against the virus contained a host-specific component which was removed by absorption with an extract from healthy tissue. The remaining antiserum was specific for the 19 .times. 50 nm virus particles.

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