Electrophysiological characterization of synaptic connections between layer VI cortical cells and neurons of the nucleus reticularis thalami in juvenile rats

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Abstract
Corticothalamic (CT) feedback projections to the thalamus outnumber sensory inputs from the periphery by orders of magnitude. However, their functional role remains elusive. CT projections may directly excite thalamic relay cells or indirectly inhibit them via excitation of the nucleus reticularis thalami (nRT), a nuclear formation composed entirely of γ‐aminobutyric acidergic neurons. The relative strengths of these two pathways will ultimately control the effects of CT projections on the output of thalamic relay cells. However, corticoreticular synapses have not yet been fully physiologically characterized. Here, local stimulation of layer VI cells by focal application of K+ or AMPA elicited excitatory postsynaptic potentials in nRT neurons with a mean peak amplitude of 2.4 ± 0.1 mV (n = 75, mean ± SEM), a mean rise time (10–90%) of 0.74 ± 0.03 ms and a weighted decay time constant of 11 ± 0.3 ms. A pharmacological profile of responses was drawn in both current‐clamp and voltage‐clamp modes, showing the presence of a small N‐methyl‐d‐aspartate receptor‐dependent component at depolarized potentials. In two pairs of synaptically coupled layer VI cell–nRT neuron, moderate rates of transmission failures were observed while the latencies were above 5 ms in both cases. Our results indicate that the corticoreticular pathway fulfills the criteria for ‘modulatory’ inputs and is temporally restricted. We suggest that it may be involved in coincidence detection of convergent corticoreticular signals.

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