Preparation and Analysis of Diagnostic Antipneumococcus Serum

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Abstract
By the use of boiled antigens the results obtained were better than those afforded by formalinized antigens. In a series of 76 rabbits injd. by this method only 1 failed to produce satisfactory Neufeld serum. This rabbit, immunized with a formalinized antigen several weeks old, yielded a serum of high species-specific agglutinin titer. The preservative is prepared by dissolving phenol crystals in an equal part of ether ("for anesthesia") and should be mixed with the serum by a gentle rotatory motion. For simplicity of application to diagnostic work, potency may be expressed as the smallest amount of a serum which will produce well defined "Quellung" of 1,000,000 homologous pneumococci.

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