Measurements of light scattering changes induced by a strong optical field in solutions of tRNA

Publisher Website
Google Scholar
Abstract
Changes in light scattering induced by a strong laser beam, as predicted theoretically by Kielich, were measured for unfractionated yeast transfer ribonucleic acid (tRNA) solutions. The vertically polarized electric field of a strong laser pulse (λ = 1060nm) amounted to 4.5 × 103 esu cgs; its duration was 10 nsec. A weak incident laser beam (λ = 630nm) was also polarized vertically and the vertical and horizontal intensity components of the light scattered through 90° at the latter wavelength were measured. These measurements together with previous results from measurements of Rayleigh light scattering and light scattering in a magnetic field permitted evaluation of the tensor of third‐order polarizability (c = 3 × 10−30 esu cgs, c = −373 × 10−30 esu cgs) and the anisotropy of the third‐order polarizability components with its sign (δc = +56 × 10−2, δc = +0.25 × 10−2 for tRNA monomer and aggregate, respectively). The new method described may be useful for studies of macromolecules and macromolecular complexes of biological importance.