Nuclear Overhauser experiments at 500 MHz on the downfield proton spectrum of a ribonuclease-resistant fragment of 5S ribonucleic acid

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Abstract
The downfield (9-15 ppm) proton NMR spectrum of a RNase A resistant fragment of Escherichia coli 5S RNA was studied by nuclear Overhauser methods. The fragment comprises bases 1-11 and 69-120 of the parent molecule. The nuclear Overhauser data identify 2 double helical structures in the fragment whose sequences are (GC)3(AU)(GC)3 and (GC)2(AU)(GU). These structures correspond exactly to the central portions of the terminal stem and prokaryotic loop helices which should exist in the fragment sequences according to the Fox-Woese model of 5S RNA secondary structure. The significance of these and other nuclear Overhauser effects detected for the structure of 5S RNA and its fragment is discussed.