High-Performance Liquid Chromatographic Measurement of Atenolol

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Abstract
A simple high-performance liquid chromatographic method was developed for the measurement of atenolol in human plasma, breast milk and urine. The sample (250 .mu.l) was vortex-mixed for 30 s with approximately 50 mg NaCl, 10 mol/l NaOH solution (50 .mu.l), internal standard solution (aqueous benzimidazole, 1.69 .mu.mol/l) (50 .mu.l) and methyl-tert-butyl ether containing 10% (vol/vol) heptafluorobutanol (200 .mu.l). After centrifugation (9950 .times. g, 2 min), a portion (100 .mu.l) of the resulting extract was analyzed on a microparticulate (5 .mu.m) silica column using methanol containing 1 mmol/l d-10-camphorsulfonic acid monohydrate as the mobile phase, and the column effluent was monitored by fluorescence detection using an excitation wavelength of 195 nm. A specimen, together with a quality control sample, can be analyzed in duplicate within 30 min. The limit of accurate measurement of the assay was 20 .mu.g/l, no endogenous sources of interference were observed and interference from other drugs was minimal.