The complete amino acid sequence of a thrombin‐like enzyme/gyroxin analogue from venom of the bushmaster snake (Lachesis muta muta)
- 23 August 1993
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 329 (1-2) , 116-120
- https://doi.org/10.1016/0014-5793(93)80205-9
Abstract
The complete amino acid sequence of a thrombin-like enzyme with gyroxin activity isolated from the venom of the bushmaster snake Lachesis muta muta was determined by automated and DABITC/PITC microsequencing of the intact protein; fragments derived from it by separate cleavages with cyanogen bromide, iodosobenzoic acid and hydroxylamine; and peptides resulting from enzymatic digestions with trypsin, pepsin, chymotrypsin, and elastase. The protein, which is composed of 228 residues, contains four putative sites of N-linked glycosylation and exhibits significant sequence similarities with other serine proteases reported from snake venoms.Keywords
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