The Application of the Orbital Sinus Bleeding Technic to Germ-Free Animals
- 1 July 1963
- journal article
- research article
- Published by Oxford University Press (OUP) in American Journal of Clinical Pathology
- Vol. 40 (1) , 46-47
- https://doi.org/10.1093/ajcp/40.1.46
Abstract
A method is described for bleeding germfree mice in Reyniers-type animal units. This employs immobilization of animals by anaesthesia to prevent accidental contamination of germfree units. Using sodium pentobarbital in a concentration of approx. .05 mgm per gram of body weight, it was possible to collect repeated blood samples up to 0.5 ml each, consistently. The orbital sinus route of bleeding under these above conditions was accompanied by no mortality.Keywords
This publication has 0 references indexed in Scilit: