Production of Granulocyte-Macrophage Colony-Stimulating Factors (GM-CSF) by Various Mammalian Cell Lines Cultured in a Protein-Free Synthetic Medium

1 January 1980

journal article
research article
Published by Japan Society for Cell Biology in Cell Structure and Function

Vol. 5 (4) , 315-321
https://doi.org/10.1247/csf.5.315

Abstract

Thirteen cell lines, all of which had been adapted to grow in a chemically defined medium, produced colony-stimulating factor (CSF), but the production rates of the factor varied considerably among the cell lines. Rat liver parenchymal, monkey kidney and rat spleen plasma cell lines were comparable to L.cntdot.P3 cells in their CSF-producing activity. Isoelectrofocusing and gel-filtration chromatography in the presence of 6 M guanidine HCl showed that the CSF of the liver and kidney cell lines resembled L.cntdot.P3 CSF, whereas the CSF of spleen cells differed from the other CSF in its molecular properties. In addition, the CSF of the spleen cell line predominantly stimulated the formation of granulocytic colonies, but the L.cntdot.P3 CSF and other CSF mainly enhanced monocytic colony formation.

This publication has 0 references indexed in Scilit: