Some Observations on the Hull Method for Measurement of Proteolysis in Milk

Abstract
Spectrophotometric readings to measure milk proteolysis by the Hull method were unreliable due to frequent appearance of turbidity. Temperatures below 26[degree]C in final reaction mixture caused turbidity. Clear filtrates resulted at higher temperatures. The difficulty in measuring bacterial proteolysis in autoclaved milk was corrected by using milk pasteurized at 62oC for 30 min.