Structure of the ARO3 gene of Saccharomyces cerevisiae
- 1 September 1988
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 214 (1) , 165-169
- https://doi.org/10.1007/bf00340197
Abstract
In Saccharomyces cerevisiae, the genes ARO3 and ARO4 encode isoenzymes of 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase. Both genes are derepressed seven-fold under the general control of amino acid biosynthesis. A previously isolated 1.7kb fragment containing the ARO3 gene and the 5′- and 3′-flanking regions was sequenced. The endpoints of the ARO3 transcript coding for a 370 amino acid protein were mapped by primer extension experiments and S1 nuclease digestion. Promoter elements involved in transcription initiation and responsible for the strong general control derepression response are discussed.Keywords
This publication has 52 references indexed in Scilit:
- Promoters, activator proteins, and the mechanism of transcriptional initiation in yeastCell, 1987
- Saturation Mutagenesis of the Yeast his3 Regulatory Site: Requirements for Transcriptional Induction and for Binding by GCN4 Activator ProteinScience, 1986
- GCN4 protein, synthesize in vitro, binds HIS3 regulatory sequences: Implications for general control of amino acid biosynthetic genes in yeastCell, 1985
- High-sensitivity S1 mapping with single-stranded [32PIDNA probes synthesized from bacteriophage M13mp templatesGene, 1984
- THE PATHWAY OF EUKARYOTIC mRNA FORMATIONAnnual Review of Biochemistry, 1983
- A short nucleotide sequence required for regulation of HIS4 by the general control system of yeastCell, 1983
- Regulatory sites for his3 gene expression in yeastNature, 1982
- The nucleotide sequence of the HIS4 region of yeastGene, 1982
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Regulation der Biosynthese der aromatischen Aminosäuren in Saccharomyces cerevisiaeEuropean Journal of Biochemistry, 1967