Studies on the Reduction of Tetrazolium Salt by Plant Tissues

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Abstract
A colorimetric method to determine the activity of TTC reduction in tissue slices of bean embryo was devised. The reduction proceeded at constant rate under aerobic conditions as well as under anaerobic conditions. The reduction rate increased with reaction temperature up to 50cC. The pH of the surrounding medium influenced the reaction rate slightly. Plasmolysis with sugar solutions depressed the reaction, whereas urea favored it at high concentration. Oeplasmolysis prior to the reaction stimulated the reduction. Plasmolysis with inorganic salt solutions influenced the reduction in different degrees. At plasmolysing concentrations, anions affected the reaction in the order of the lyotropic series. Relationship between the activity of the reaction and site of the enzymes in the cytoplasm and its colloidal state is discussed.

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