Immune Response to Gross Virus-Induced Lymphoma: Comparison of Two In Vitro Assays of Cell-Mediated Immunity

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Abstract
A microcytotoxicity assay (MCA) and a 51chromium-release cytotoxicity assay (CRA) for cell-mediated immunity were used to study the response of W/Fu rats to the Gross virus-induced lymphoma (C58NT)D. Generally, there was good correlation between the results of the 2 assays. At 10 days after tumor inoculation, positive direct reactivity was detected in both MCA and CRA. At 40 days after immunization, MCA was positive but direct CRA was negative. However, in vitro activation of immune lymphocytes by preincubation for 24 hours at 37° C gave positive results in CRA. With in vitro activation, cytotoxic effects in CRA were found with lymphocytes from rats inoculated as long as 180 days before. With MCA, positive results were obtained for at least 120 days after tumor inoculation. Both assays also gave positive results when rats, injected neonatally with Gross leukemia virus, were tested at 60 days of age. It appears likely that the 2 assays reflect the same basic immune response to tumor-associated cell surface antigen.

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