Intermolecular and intramolecular isotope effects in the deamination of putrescine catalyzed by diamine oxidase
- 1 April 1982
- journal article
- research article
- Published by Springer Nature in Cellular and Molecular Life Sciences
- Vol. 38 (4) , 431-433
- https://doi.org/10.1007/bf01952622
Abstract
The enzymatic deamination of 1,4-diaminobutane (putrescine) catalyzed by hog kidney diamine oxidase was studied with the aid of deuterium labeled substrates and mass spectrometry. An intermolecular deuterium isotope effect for the deamination of putrescine labeled with deuterium in all 4 alpha positions was observed to be 1.26. 1,4-Diaminobutane-1, 1-d2 was synthesized and intramolecular isotope effects determined. The preference of diamine oxidase for the unlabeled alpha position was about 4 times greater than for the deuterated methylene. This work shows that intramolecular deuterium isotope effects are observable in enzyme systems other than cytochrome P-450.Keywords
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