Three‐Dimensional View Of The Chromatin In Freeze‐Fractured Chicken Erythrocyte Nuclei

Publisher Website
Google Scholar
Abstract
The previously reported freeze-fracture thaw-fix technique was applied to detailed study of the chicken erythrocyte, by transmission replicas and high resolution scanning electron microscopy (3 nm scan beam size). The 3-dimensional structure of chromatin, and possibly the non-histone protein matrix, of fractured nuclei was to a large extent retained in this method of preparation and seen in stereomicrographs. The helical sub-structure of the 25 nm chromatin strands was seen at about the same resolution as that of previously published micrographs in which extracted chromatin was viewed by negative contrast or after metal shadowing. The useful resolution of the secondary EM, for a suitably mounted specimen, is as good as that of transmission electron micrographs of PC replicas of the same material.