RNA primer used in synthesis of anticomplementary DNA by reverse transcriptase of avian myeloblastosis virus.

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Abstract
When either the homologous RNA (avian myeloblastosis virus RNA) or a heterologous RNA (poliovirus RNA) was used as a template, the anticomplementary DNA synthesized in vitro by avian myeloblastosis virus reverse transcriptase (RNA-directed DNA nucleotidyltransferase, EC 2.7.7.7) was primed by fragments of the original RNA template that usually had adenosine at their 3'' ends. When phage T4 RNA ligase (EC 6.5.1.3) was used to label the 3'' end of the RNA template fragments contained in the RNA .cntdot. c[complementary]DNA hybrid intermediate, adenosine was the principal nucleoside carrying the label. The RNase H (hybrid nuclease) activity of the reverse transcriptase creates fragments of the original RNA template with adenosine as the principal 3'' terminus and that these fragments serve as primers for the synthesis of anticomplementary DNA.