The application of 13C NMR to the characterization of phospholipid metabolism in cells

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Abstract
31P and 31C NMR spectroscopy of lipid extracts of T47D human breast cancer spheroids and the use of 13C‐labeled lipid precursors [3‐13C ] serine, [1,2‐13C] ethanolamine, and [1,2‐13C]choline enabled us to determine the rate of 13C incorporation into the major phos‐pholipids and to show that the synthesis of phosphatidylethanolarnine in T47D cells is via both the CDP‐ethanolamine pathway and serine decarboxylation, with the extent of each depending on the concentration of ethanolamine in the medium. In the presence of low ethanolamine (3.4 μ M), both pathways contribute in equal proportions, while in the presence of high ethanolamine, the CDP‐ethanolamine pathway predominates. © 1992 Academic Press, Inc.