Valyl‐tRNA, Isoleucyl‐tRNA and Tyrosyl‐tRNA Synthetase from Baker's Yeast
- 1 July 1976
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 66 (3) , 493-497
- https://doi.org/10.1111/j.1432-1033.1976.tb10574.x
Abstract
Nineteen analogs of ATP have been tested in the aminoacylation reaction of valyl‐tRNA, isoleucyl‐tRNA and tyrosyl‐tRNA synthetases from baker's yeast. Four compounds are substrates for valyl‐tRNA and two for isoleucyl‐tRNA synthetase, but there is no modified substrate for the tyrosyl‐tRNA synthetase. There is one inhibitor for valyl‐tRNA synthetase, eight compounds inhibit isoleucyl‐tRNA synthetase and two compounds inhibit tyrosyl‐tRNA synthetase. Their Km and Kt and V values have been determined.The substrate specificity shows that positions 2, 6, 7, 8, 9, 2′, and 3′ of ATP are important for catalytic action of these aminoacyl‐tRNA synthetases.This publication has 25 references indexed in Scilit:
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