Voltage‐dependent Ca2+ channel subunit expression and immunolocalization in mouse spermatogenic cells and sperm
Open Access
- 22 November 1999
- journal article
- Published by Wiley in FEBS Letters
- Vol. 462 (1-2) , 171-176
- https://doi.org/10.1016/s0014-5793(99)01518-5
Abstract
Though voltage‐dependent Ca2+ channels contribute to the orchestratation of sperm differentiation and function, many questions remain concerning their molecular architecture. This study shows that α1A and α1C Ca2+ channel pore‐forming subunits are expressed in spermatogenic cells. In addition, it provides what is to our knowledge the first evidence for the presence of the Ca2+ channel β auxiliary subunits in spermatogenic cells and sperm. Using RT‐PCR we demonstrated the expression of all four known genes encoding the β subunits in spermatogenic cells. Specific antibodies detected three of these proteins in spermatogenic cells and sperm. In spermatogenic cells both α1 and β subunits are diffusely distributed throughout the cytoplasm while in sperm they appear to be regionally localized.Keywords
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