Protection from microbial contamination in a room ventilated by a uni-directional air flow

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Abstract
Experiments have been carried out on the extent to which movement of persons in a room ventilated by a horizontally directed uniform air velocity can transport airborne bacteria from one position to another. More than 107 particles approximately 13 μ in diameter (settling rate in air about 1 ft./min.) carrying spores of Bacillus subtilis var. niger were liberated in each experiment and the numbers reaching the different parts of the room were estimated by those recovered on exposed settling plates. At air velocities of 35 ft./min. and over, no particles could be certainly found to have moved against the direction of air flow except into areas actually entered by persons, and the numbers found in these areas were between 100 and 1000 times less than would have been expected in rooms turbulently ventilated with the same volume of air. There was some transport of particles transversely across the air-flow lines but at 4 ft. distance from the area where movement of persons was taking place the numbers had fallen substantially below 1/100 of those to be expected in a turbulently ventilated room.The results recorded at an air velocity of 22 ft./min. were significantly less satisfactory.Our thanks are due to the N.W. Metropolitan Regional Hospital Board, to the Hospital Management Committee of St Charles' Hospital, London, W10, and especially to the hospital engineer for accommodating the experimental room and facilitating its operation.