mRNA-dependent in vitro synthesis of ribosomal proteins L12 and L10 and elongation factor Tu.
- 1 December 1977
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 74 (12) , 5387-5391
- https://doi.org/10.1073/pnas.74.12.5387
Abstract
RNA extracted from growing Escherichia coli can direct the in vitro synthesis of ribosomal proteins L12 and L10 and elongation factor Tu when an E. coli system is used. The synthesized L12 can be bound to L12-depleted ribosomes and the synthesized elongation factor Tu can form complexes with both elongation factor Ts and GDP. Guanosine 5''-diphosphate 3''-diphosphate has no effect on the synthesis of these proteins from an RNA template but inhibits their synthesis when a DNA template is used.This publication has 47 references indexed in Scilit:
- DNA-dependent in vitro synthesis of Escherichia coli ribosomal protein L10 and the formation of an L10L12 complexBiochemical and Biophysical Research Communications, 1977
- DNA-directed in vitro synthesis of elongation factor TuBiochemical and Biophysical Research Communications, 1976
- DNA-dependent in vitro synthesis of ribosomal proteins, protein elongation factors, and RNA polymerase subunit α: Inhibition by ppGppCell, 1976
- Pools of Ribosomal Proteins in Escherichia coli. Studies on the Exchange of Proteins between Pools and RibosomesEuropean Journal of Biochemistry, 1975
- The identification and characterization of proteins similar to L7,L12 in ribosome-free extracts of escherichia coliBiochemical and Biophysical Research Communications, 1975
- Stringent control of the transcriptional activities of ribosomal protein genes in E. coliNature, 1975
- The messenger-directed synthesis of the α-fragment of the enzyme β-galactosidaseBiochemical and Biophysical Research Communications, 1973
- 50-S Ribosomal Proteins. Purification and Partial Characterization of Two Acidic Proteins, A1 and A2, Isolated from 50-S Ribosomes of Escherichia coliEuropean Journal of Biochemistry, 1972
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- In vitro Synthesis of Bacteriophage LysozymeNature, 1967