FUNCTIONAL PRESERVATION OF THE MAMMALIAN KIDNEY

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Abstract
This research demonstrates that dog kidneys perfused with dimethylsulfoxide (Me2SO) in likely cryoprotective concentrations (2.8 M) can survive as the sole source of renal support when autologously transplanted. Kidneys were perfused in vitro with Me2SO in one of two vehicles: solution A (K+-Mg2+-rich) or RPS-2 (K+-glucose-rich). Me2SO concentration in the vehicle was gradually increased to maximum (2.8 or 4.2 M) over a period of 28 to 35 min, held for 5-10 min, then decreased over 55-65 min. All groups except one consisted of 5 kidneys perfused at 25.degree. C. Survivors were dogs living 21 days postoperatively. The first group received kidneys perfused with solution A; 5 dogs survived, serum creatinine on day 21 [Cr], [mean .+-. SE] = 1.3 .+-. 0.1. The second group received RPS-2 kidneys; 4 survived, Cr = 1.4 .+-. 0.1. The third group received solution A kidneys with 2.8 M Me2SO; 3 survived, Cr = 2.7 .+-. 1.3. The fourth group received RPS-2 kidneys with 2.8 M Me2SO; 5 survived, Cr = 1.2 .+-. 0.2. The fifth group received RPS-2 kidneys with 4.2 M Me2SO; 2 survived, Cr = 1.7 .+-. 0.3. One group of 6 dogs received kidneys perfused at 10.degree. C with 4.2 M Me2SO in RPS-2; one survived, Cr = 1.4. Results demonstrate beneficial interaction of vehicle with Me2SO and the efficiacy of 25.degree. C perfusion.