A Resinous Mounting Medium Compatible with Azo-Dye Staining for Alkaline Phosphatase
- 1 January 1966
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 41 (5) , 295-297
- https://doi.org/10.3109/10520296609116326
Abstract
Thick sections, 75-125 μ, are required for adequate demonstration of blood vessels and occasional other structures which show alkaline phosphatase activity. The mounting medium described has been found to be superior for thick sections to others which we have used such as polyvinylpyrrolidone, polyvinylacetate or glycerol-gelatin. The mounting technique consists of dehydration in absolute ethanol, one change of equal parts of ethanol and cumene, clearing in ethyl benzene, and mounting with synthetic resin dissolved in ethyl benzene.This publication has 1 reference indexed in Scilit:
- Histochemical Comparison of Naphthol AS-Phosphates for the Demonstration of PhosphatasesJNCI Journal of the National Cancer Institute, 1958