Enrichment of human heterokaryons by ficoll gradient for complementation analysis of iduronate sulfatase deficiency
- 1 November 1979
- journal article
- research article
- Published by Springer Nature in Somatic Cell and Molecular Genetics
- Vol. 5 (6) , 1079-1089
- https://doi.org/10.1007/bf01542661
Abstract
Ficoll gradients have been used to enrich for heterokaryons in cultures of human skin fibroblasts following polyethylene glycol (PEG) induced fusion. These gradients provide a simple and consistent method for obtaining populations of multinucleated cells, at least twofold greater than those resulting from fusion alone. Formation of glucose-6-phosphate dehydrogenase (G6PD) heteropolymers has been used as a functional assay for the presence of heterokaryons. Analysis of cell populations enriched for multinucleated cells has revealed complementation leading to iduronate sulfatase activity in heterokaryons derived from iduronate sulfatase-deficient fibroblasts expressing the Hunter and multiple sulfatase-deficiency mutations.This publication has 14 references indexed in Scilit:
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