THE PRODUCTION OF NEURAMINIDASE BY FOOD-POISONING STRAINS OF CLOSTRIDIUM WELCHII (C. PERFRINGENS)

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Abstract
The production of neuraminidase by a classical strain of Clostridium welchii (C. perfringens) type A was studied. Good yields were produced in 5% Proteose Peptone-water medium (PPW5); the enzyme was essentially extracellular but some further neuraminidase could be released by ultrasonic disintegration of the cells. This also released N-acyl neuraminic acid-aldolase (NAN-aldolase) and the degree to which this interferes with the assay for neuraminidase was evaluated.

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