Oligonucleotide Studies

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Abstract
Four dinucleotides and eight trinucleotides were obtained on a preparative scale from the pancreatic RNase [EG 2.7.7.16] digestion of commercially available yeast RNA and its subsequent chromatographic separation with DEAE-Sephadex A-25 followed by AG 1-×2 (or Dowex 1-×2). The AG 1-×2 (or Dowex 1-×2) column employed in the separation of the di- and trinucleotide fractions according to base sequence showed satisfactory results at pH 2.2. Since a pair of the sequence isomers ApGpGp and GpApGp were eluted in a single peak, these isomers were separated on a DEAE-cellulose column (formate form). Present chromatographic procedures are suited to the large scale preparation of pure di- and trinucleotides which is a prerequisite to further study of oligonucleotides. Optical rotatory dispersion (ORD) and circular dichroic spectra of ApGpGp and GpApCp*** were measured at pH7 to show their dependence on the base sequence. The results of fractionation of the tetranucleotides obtained from digestion of RNA with RNase T1, followed by pancreatic RNase digestion, are also included in the Appendix. The spectrophotometric constants were determined for these tetranucleotides; ApApApCp, ApApApGp and ApAp ApUp.

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