areCBA Is an Operon in Acinetobacter sp. Strain ADP1 and Is Controlled by AreR, a ς 54 -Dependent Regulator
Open Access
- 1 January 2001
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 183 (1) , 6045
- https://doi.org/10.1128/jb.183.1.405-409.2001
Abstract
The areCBA genes in Acinetobacter sp. strain ADP1, determining growth on benzyl alkanoates, are shown to be transcribed as a single operon and regulated by areR , which encodes a regulatory protein of the NtrC/XylR family. Assays of the Are enzymes and of two insertions of lacZ as a reporter gene have shown that the operon is induced by benzyl acetate, benzyl alcohol, and benzaldehyde, as well as 2- and 4-hydroxybenzyl acetates and benzyl propionate and butyrate. Two adjacent sites of transcriptional initiation were 97 and 96 bp upstream of the start codon for areC , near a ς 54 -dependent −12, −24 promoter. Inactivation of areR and rpoN (for RNA polymerase ς 54 ) drastically reduced growth rates on the Are substrates and induction of the operon.Keywords
This publication has 26 references indexed in Scilit:
- xyIUW, Two Genes at the Start of the upper Pathway Operon of TOL Plasmid Pwwo, Appear to Play no Essential Part in Determining its Catabolic PhenotypeMicrobiology, 1997
- THE β-KETOADIPATE PATHWAY AND THE BIOLOGY OF SELF-IDENTITYAnnual Review of Microbiology, 1996
- Signal sensing by σ54‐dependent regulators: derepression as a control mechanismMolecular Microbiology, 1996
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994
- In a class of its own — the RNA polymerase sigma factor σ;54 (σN)Molecular Microbiology, 1993
- Specific binding of the transcription factor sigma-54 to promoter DNANature, 1992
- Basic local alignment search toolJournal of Molecular Biology, 1990
- Construction of a lacZ-kanamycin-resistance cassette, useful for site-directed mutagenesis and as a promoter probeGene, 1989
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- The Growth of Micro-organisms in Relation to their Energy SupplyMicrobiology, 1960