Granulocyte‐colony stimulating factor maintains a thermally stable, compact, partially folded structure at pH 2

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Abstract
At acidic pH many proteins exist in a partially unfolded form, called the “A” state. This is defined as a flexible, expanded structure with well‐defined, usually native‐like secondary structure, but no unique tertiary structure, and showing no cooperativity during thermal‐induced denaturation. Granulocyte‐colony stimulating factor (G‐CSF), a four‐helix bundle cytokine, maintains both thermal stability and tertiary structure at pH 2.O. We therefore examined the conformation and thermal unfolding of G‐CSF at pH 2.0, 4.0 and 7.0 using circular dichroism (CD) and Fourier transform infrared spectroscopy (FTIR). The secondary structure of the molecule remains highly helical as the pH is lowered from 7.0 to 2.0. The tertiary structure of the protein is slightly different at each pH value, but even at pH 2.0 G‐CSF maintains a regular three‐dimensional structure. The structure is hydrodynamically compact at these different pH values, with no increase in Stake's radius even at pH 2.0.The thermal‐induced denaturation of G‐CSF was determined by monitoring changes in the CD or FTIR spectra. At pH 2.0 the temperature at which thermal‐induced denaturation begins is higher than it is at pH 4.0 or 7.0, the thermal unfolding transition remains cooperative and some α‐helical structure persists even at 86°C. At pH 4.0 and 7.0, secondary and tertiary structures disappear simultaneously during thermal denaturation, whereas at pH 2.0 small changes in the far‐UV CD region begin to occur first, followed by the simultaneous cooperative loss of tertiary structure and much of the remaining secondary structure. The structure of G‐CSF at pH 2.0 is thus revealed as compact, with a unique, three‐dimensional structure, highly helical secondary structure, and most importantly, a cooperative thermal unfolding transition. G‐CSF at acid pH thus does not adopt the “A” state.