Cell Survival Measurements at Low Doses Using an Automated Image Cytometry Device

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Abstract
The statistical precision of cell survival data measured at low doses can be greatly improved if the number of cells assayed at each dose point is known exactly. This paper describes an image cytometry technique that has been developed for this purpose. Treated cells are plated into tissue culture flasks, and after attachment their locations are determined with 98% accuracy by automated scanning procedures. These locations are revisited after an appropriate incubation period to assay for colony formation. Colonies may be scored either manually or using image data collected by the device. Additional information, such as heterogeneity of colony size, can also be obtained.