Tissue Progesterone Assay by Means of Competitive Protein Binding
- 12 January 1972
- journal article
- Published by Wiley in Acta Obstetricia et Gynecologica Scandinavica
- Vol. 51 (2) , 117-120
- https://doi.org/10.3109/00016347209154005
Abstract
An assay technique is described which permits the estimation of nanogram amounts of progesterone in tissues. After primary extraction of the tissue homogenate, the extract is purified by means of thin layer and column chromatography. Progesterone is then measured by competitive protein binding. With this technique it is possible to determine the amount of progesterone in less than 1 gram of myometrial or endometrial tissue.Keywords
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