Chimeric 16S rDNA sequences of diverse origin are accumulating in the public databases
- 1 January 2003
- journal article
- Published by Microbiology Society in International Journal of Systematic and Evolutionary Microbiology
- Vol. 53 (1) , 289-293
- https://doi.org/10.1099/ijs.0.02441-0
Abstract
A significant number of chimeric 16S rDNA sequences of diverse origin were identified in the public databases by partial treeing analysis. This suggests that chimeric sequences, representing phylogenetically novel non-existent organisms, are routinely being overlooked in molecular phylogenetic surveys despite a general awareness of PCR-generated artefacts amongst researchers.Keywords
This publication has 29 references indexed in Scilit:
- Geochemistry and microbial diversity of a trichloroethene-contaminated Superfund site undergoing intrinsic in situ reductive dechlorinationFEMS Microbiology Ecology, 2002
- Characterization of a 4-methylbenzoate-degrading methanogenic consortium as determined by small-subunit rDNA sequence analysisJournal of Bioscience and Bioengineering, 2001
- Archaeal Diversity in Waters from Deep South African Gold MinesApplied and Environmental Microbiology, 2001
- Bacteria and Archaea Physically Associated with Gulf of Mexico Gas HydratesApplied and Environmental Microbiology, 2001
- Characterization of microbial consortia in a terephthalate-degrading anaerobic granular sludge system The GenBank accession numbers for the sequences obtained in this work are AF229774–AF229793.Microbiology, 2001
- Evaluation of PCR-Generated Chimeras, Mutations, and Heteroduplexes with 16S rRNA Gene-Based CloningApplied and Environmental Microbiology, 2001
- The RDP-II (Ribosomal Database Project)Nucleic Acids Research, 2001
- Microvariation Artifacts Introduced by PCR and Cloning of Closely Related 16S rRNA Gene SequencesApplied and Environmental Microbiology, 2001
- The frequency of chimeric molecules as a consequence of PCR co-amplification of 16S rRNA genes from different bacterial speciesMicrobiology, 1996
- Potential risks of gene amplification by PCR as determined by 16S rDNA analysis of a mixed-culture of strict barophilic bacteriaMicrobial Ecology, 1991