Comparison of Different Assay Methods for Determination of Elastase Activity

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Abstract
Four different methods of pancreatic elastase (E. C. 3.4.21.11) assay have been compared: one use N-succinyl-(Ala)3-paranitroanilide as a substrate; the three others use elastin, modified or not. The former has been monitored either with a spectrophotometric or a conductimetric device. All methods give a linear relationship with amounts of pancreatic elastase in the range 0–5 μg. The conductimetric method is the only one using unmodified elastin which measures elastolysis in initial rate conditions, avoiding artificial amplification of hydrolysis if other proteases are present.