Expression of the enkephalin precursor gene in rat Sertoli cells
- 12 September 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 237 (1-2) , 183-186
- https://doi.org/10.1016/0014-5793(88)80197-2
Abstract
Searching for somatic cells expressing the preproenkephalin (A) gene in the testis, we have isolated Sertoli cells from the testes of 20-day-old rats. Cultured Sertoli cells contained a single species (about 1.5 kb) of preroenkephalin mRNA, and follicle-stimulating hormone (FSH) transiently increased the mRNA abundance to a maximum (about 30 molecules per cell) at 12 h. Various compounds that activate the cyclic AMP system in Sertoli cells similarly increased the abundance of preproenkephalin mRNA. Moreover, FHS increased intracellular Met-enkephalin immunoreactive peptides in Sertoli cells. Thusthe preproenkephalin gene expression in Sertoli is positvely regulated by FSH through the cyclic AMP system.Keywords
This publication has 18 references indexed in Scilit:
- Enkephalin precursor gene expression in postmeiotic germ cellsBiochemical and Biophysical Research Communications, 1988
- β-Endorphin regulation of FSH-stimulated inhibin production is a component of a short loop system in testisBiochemical and Biophysical Research Communications, 1987
- Differential expression of opioid peptide genes by testicular germ cells and somatic cells.Proceedings of the National Academy of Sciences, 1987
- Glucocorticoids and cyclic AMP synergistically regulate the abundance of preproenkephalin messenger RNA in neuroblastoma-glioma hybrid cellsBiochemical and Biophysical Research Communications, 1986
- Expression of the enkephalin precursor gene in C6 rat glioma cells: Regulation by β-adrenergic agonists and glucocorticoidsMolecular Brain Research, 1986
- Activation of Mouse T-Helper Cells Induces Abundant Preproenkephalin mRNA SynthesisScience, 1986
- Measurement of protein using bicinchoninic acidAnalytical Biochemistry, 1985
- Biochemistry of the enkephalins and enkephalin-containing peptidesArchives of Biochemistry and Biophysics, 1983
- Cell-free translation and partial characterization of mRNA coding for enkephalin-precursor protein.Proceedings of the National Academy of Sciences, 1982
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979