Immunochemical Measurement of Conformational Heterogeneity of Poly(Inosinic Acid)

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Abstract
Several pure poly(I) preparations differed in their complement fixation reactivity with [rabbit] antipoly(I) antiserum; their ability to bind to a solid-phase anti-poly(I) antibody-Sepharose column; their ability to inactivate serum complement; and their reactivity with purified antibodies to double-stranded RNA. In particular, poly(I) samples that could induce interferon production [primary rabbit kidney cells] differed from non-inducer poly(I)s; the inducers reacted weakly with anti-poly(I) antiserum and were the only ones that reacted with antibodies to double-stranded RNA. One inducer poly(I) did not inactivate complement, and differed from non-inducer poly(I) in quantitative aspects of poly(I) .cntdot. poly(C) formation with varying amounts of poly(C). An additional type of poly(I) preparation reacted poorly with anti-poly(I) antiserum, did not react with anti-double-stranded-RNA antibodies and failed to induce interferon production. The varying forms of poly(I) were not interconvertible by boiling and rapid chilling. Several different stable structural forms of poly(I) apparently may result from a standardized synthetic procedure.