STUDIES ON IMMUNITY TO TOXINS OFCLOSTRIDIUM BOTULINUMIV

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Abstract
A medium was developed for the production of C. botulinum type C toxin. The medium contained 4% proteose peptone, 2% N-Z-Amine-type B, 2% yeast extract, and 1% glucose. Toxicities of approximately 1,000,000 mouse intraperitoneal LD50 per ml were produced in 5 days at 33[degree]C. The toxin was purified by a procedure that included either alcohol or ammonium sulfate precipitation, followed by a calcium chloride extraction and an additional alcohol precipitation. Specific activity of the purified toxin was 20 to 60 x 106 LD50 per mg N. Toxin was converted to toxoid in the presence of 0.6% formalin at 33[degree]C and after adsorption on aluminum phosphate was highly antigenic for mice and guinea pigs.