Optimization of Real-Time PCR Assay for Rapid and Sensitive Detection of Eubacterial 16S Ribosomal DNA in Platelet Concentrates
- 1 October 2003
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 41 (10) , 4796-4798
- https://doi.org/10.1128/jcm.41.10.4796-4798.2003
Abstract
A real-time PCR assay was developed for rapid detection of eubacterial 16S ribosomal DNA in platelet concentrates. The sensitivity of this assay can be hampered by contaminating DNA in the PCR reagents. Digestion of the PCR reagents with Sau3AI prior to PCR amplification was effective in eliminating this contaminating DNA without affecting the sensitivity of the assay.Keywords
This publication has 19 references indexed in Scilit:
- Contamination Management of Broad-Range Ribosomal DNA PCR: Where Is the Evidence?Journal of Clinical Microbiology, 2002
- Quantitative Multiprobe PCR Assay for Simultaneous Detection and Identification to Species Level of Bacterial PathogensJournal of Clinical Microbiology, 2002
- Risk Assessment Models and Contamination Management: Implications for Broad-Range Ribosomal DNA PCR as a Diagnostic Tool in Medical BacteriologyJournal of Clinical Microbiology, 2002
- Determination of bacterial load by real-time PCR using a broad-range (universal) probe and primers setMicrobiology, 2002
- Bacterial contamination of platelet concentrates: Incidence, significance, and preventionSeminars in Hematology, 2001
- Bacterial contamination of platelet concentrates: Incidence, significance, and preventionSeminars in Hematology, 2001
- Approaches to the detection of bacterial contamination in cellular blood productsTransfusion Medicine Reviews, 1999
- Decontamination of polymerase chain reaction reagents for detection of low concentrations of 16S rRNA genesMolecular Biotechnology, 1997
- Blood Product-Associated Bacterial SepsisTransfusion Medicine Reviews, 1991
- Avoiding false positives with PCRNature, 1989