Crystal structure of a bacteriophage T7 DNA replication complex at 2.2 Å resolution
- 15 January 1998
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 391 (6664) , 251-258
- https://doi.org/10.1038/34593
Abstract
DNA polymerases change their specificity for nucleotide substrates with each catalytic cycle, while achieving error frequencies in the range of 10−5to 10−6. Here we present a 2.2 Å crystal structure of the replicative DNA polymerase from bacteriophage T7 complexed with a primer–template and a nucleoside triphosphate in the polymerase active site. The structure illustrates how nucleotides are selected in a template-directed manner, and provides a structural basis for a metal-assisted mechanism of phosphoryl transfer by a large group of related polymerases.Keywords
This publication has 50 references indexed in Scilit:
- Crystal structure of a thermostable Bacillus DNA polymerase l large fragment at 2.1 Å resolutionStructure, 1997
- [20] Processing of X-ray diffraction data collected in oscillation modePublished by Elsevier ,1997
- A Thumb Subdomain Mutant of the Large Fragment of Escherichia coli DNA Polymerase I with Reduced DNA Binding Affinity, Processivity, and Frameshift FidelityJournal of Biological Chemistry, 1996
- Structure of Taq polymerase with DNA at the polymerase active siteNature, 1996
- Significance of the O-Helix Residues of Escherichia coli DNA Polymerase I in DNA Synthesis: Dynamics of the dNTP Binding PocketBiochemistry, 1996
- Deoxynucleoside Triphosphate and Pyrophosphate Binding Sites in the Catalytically Competent Ternary Complex for the Polymerase Reaction Catalyzed by DNA Polymerase I (Klenow Fragment)Published by Elsevier ,1995
- Crystal structures of the Klenow fragment of DNA polymerase I complexed with deoxynucleoside triphosphate and pyrophosphateBiochemistry, 1993
- SETOR: Hardware-lighted three-dimensional solid model representations of macromoleculesJournal of Molecular Graphics, 1993
- An attempt to unify the structure of polymerasesProtein Engineering, Design and Selection, 1990
- Elementary steps in the DNA polymerase I reaction pathwayBiochemistry, 1983