THE CARDIAC CALCIUM PUMP

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Abstract
A granular fraction derived from rabbit hearts by fragmentation of the endoplasmie reticulum was partially purified by gradient centrifugation in sucrose. This sarcotubular fraction rapidly accumulated Ca in the presence of oxalate, Mg++, and ATP. There was an increase in ATP splitting over that observed in the absence of Ca, with 1 or 2 [image] of Ca taken up for one [image] ATP extra-split. Calcium uptake as well as ATP splitting were inhibited by p-choloromercuribenzoate and by Salyrgan pointing to the involvement of an enzyme with active sulfhydryl groups. This system may well be active in excitation-contraction coupling.

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